PrimePCR™ SYBR® Green Assay: SDHB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050983

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043721
Assay Design:   exonic
Chromosome Location:   1:17345335-17349109question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000375499

Gene Information

Complex II of the respiratory chain which is specifically involved in the oxidation of succinate carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq Jul 2008]

Gene Symbol:   SDHB
Gene Name:   succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Aliases:   FLJ92337, IP, PGL4, SDH, SDH1, SDH2, SDHIP
RefSeq:   NC_000001.10 NG_012340.1 NT_004610.19
Ensembl:   ENSG00000117118
Entrez:   6390
UniGene:   Hs.465924
Chromosome Mapping:   1p36.1-p35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.740000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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