PrimePCR™ SYBR® Green Assay: LPHN2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050982

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LPHN2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LPHN2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043720
Assay Design:   exonic
Chromosome Location:   1:82456899-82457006question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000370721 ENST00000370727 ENST00000370725 ENST00000370723 ENST00000370728 ENST00000370730 ENST00000359929 ENST00000370715 ENST00000370713 ENST00000319517 ENST00000449420 ENST00000402328 ENST00000370717 ENST00000394879 ENST00000271029 ENST00000335786

Gene Information

This gene encodes a member of the latrophilin subfamily of G-protein coupled receptors (GPCR). Latrophilins may function in both cell adhesion and signal transduction. In experiments with non-human species endogenous proteolytic cleavage within a cysteine-rich GPS (G-protein-coupled-receptor proteolysis site) domain resulted in two subunits (a large extracellular N-terminal cell adhesion subunit and a subunit with substantial similarity to the secretin/calcitonin family of GPCRs) being non-covalently bound at the cell membrane. While several transcript variants have been described the biological validity of only one has been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   LPHN2
Gene Name:   latrophilin 2
Aliases:   CIRL2, CL2, LEC1, LPHH1
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000117114
Entrez:   23266
Chromosome Mapping:   1p31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.100000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download