PrimePCR™ SYBR® Green Assay: PASK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050898

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043636
Assay Design:   exonic
Chromosome Location:   2:242076499-242077377question
Amplicon Length:   161
Splice Variants Targeted:   ENST00000395843 ENST00000361200 ENST00000271385 ENST00000443333 ENST00000234040 ENST00000405260 ENST00000358649 ENST00000403638 ENST00000433589 ENST00000415234 ENST00000544142 ENST00000539818 ENST00000543189 ENST00000536444 ENST00000544627 ENST00000540793 ENST00000261168

Gene Information

This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Nov 2011]

Gene Symbol:   PASK
Gene Name:   PAS domain containing serine/threonine kinase
Aliases:   DKFZp434O051, DKFZp686P2031, KIAA0135, PASKIN, STK37
RefSeq:   NC_000002.11 NT_005416.13
Ensembl:   ENSG00000115687
Entrez:   23178
UniGene:   Hs.397891
Chromosome Mapping:   2q37.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.300000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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