PrimePCR™ SYBR® Green Assay: ST3GAL5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050884

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043622
Assay Design:   exonic
Chromosome Location:   2:86088401-86090606question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000525834 ENST00000455892 ENST00000377332 ENST00000393805 ENST00000393808

Gene Information

Ganglioside GM3 is known to participate in the induction of cell differentiation modulation of cell proliferation maintenance of fibroblast morphology signal transduction and integrin-mediated cell adhesion. The protein encoded by this gene is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. The encoded protein is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus. Mutation in this gene has been associated with Amish infantile epilepsy syndrome. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ST3GAL5
Gene Name:   ST3 beta-galactoside alpha-2,3-sialyltransferase 5
Aliases:   SATI, SIAT9, SIATGM3S, ST3GalV
RefSeq:   NC_000002.11 NT_022184.15 NG_012807.1
Ensembl:   ENSG00000115525
Entrez:   8869
UniGene:   Hs.415117
Chromosome Mapping:   2p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.310000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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