PrimePCR™ SYBR® Green Assay: IGFBP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0050877

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043615
Assay Design:   exonic
Chromosome Location:   2:217498661-217525398question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000434997 ENST00000233809 ENST00000456764

Gene Information

Description Not Available

Gene Symbol:   IGFBP2
Gene Name:   insulin-like growth factor binding protein 2, 36kDa
Aliases:   IBP2, IGF-BP53
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000115457
Entrez:   3485
UniGene:   Hs.438102
Chromosome Mapping:   2q33-q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.580000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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