PrimePCR™ SYBR® Green Assay: LANCL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050868

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LANCL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LANCL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043606
Assay Design:   exonic
Chromosome Location:   2:211306167-211319943question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000443314 ENST00000441020 ENST00000450366 ENST00000233714 ENST00000431941 ENST00000448951

Gene Information

This gene encodes a loosely associated peripheral membrane protein related to the LanC family of bacterial membrane-associated proteins involved in the biosynthesis of antimicrobial peptides. This protein may play a role as a peptide-modifying enzyme component in eukaryotic cells. Previously considered a member of the G-protein-coupled receptor superfamily this protein is now in the LanC family. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Nov 2008]

Gene Symbol:   LANCL1
Gene Name:   LanC lantibiotic synthetase component C-like 1 (bacterial)
Aliases:   GPR69A, p40
RefSeq:   NC_000002.11 NG_008285.1 NT_005403.17
Ensembl:   ENSG00000115365
Entrez:   10314
Chromosome Mapping:   2q33-q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.430000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download