PrimePCR™ SYBR® Green Assay: RPS15, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050852

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: RPS15, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RPS15, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043590
Assay Design:   exonic
Chromosome Location:   19:1440118-1440242question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000537330 ENST00000544334 ENST00000306801 ENST00000570891 ENST00000511885 ENST00000511098 ENST00000382865 ENST00000294072 ENST00000426130 ENST00000325351 ENST00000591479 ENST00000586686 ENST00000593052 ENST00000586096 ENST00000589656 ENST00000585665 ENST00000282869 ENST00000252242 ENST00000574902 ENST00000396878 ENST00000576416 ENST00000572647 ENST00000416391 ENST00000267620 ENST00000392414 ENST00000361409 ENST00000356592 ENST00000414552 ENST00000361925 ENST00000393933 ENST00000571194 ENST

Gene Information

Ribosomes the organelles that catalyze protein synthesis consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm. This gene has been found to be activated in various tumors such as insulinomas esophageal cancers and colon cancers. As is typical for genes encoding ribosomal proteins there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq Jul 2008]

Gene Symbol:   RPS15
Gene Name:   ribosomal protein S15
Aliases:   MGC111130, RIG, S15
RefSeq:   NC_000019.9 NG_017014.1 NT_011255.14
Ensembl:   ENSG00000115268
Entrez:   6209
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.984000
y-intercept 35.870000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download