PrimePCR™ SYBR® Green Assay: GCG, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050851

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043589
Assay Design:   exonic
Chromosome Location:   2:163008733-163008846question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000418842 ENST00000375497

Gene Information

The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these glucagon is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally the fourth peptide is similar to glicentin an active enteroglucagon. [provided by RefSeq Jul 2008]

Gene Symbol:   GCG
Gene Name:   glucagon
Aliases:   GLP1, GLP2, GRPP
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000115263
Entrez:   2641
UniGene:   Hs.516494
Chromosome Mapping:   2q36-q37

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.690000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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