This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0050827
PrimePCR™ PreAmp for SYBR® Green Assay: PLCH1, Human
PrimePCR™ Template for SYBR® Green Assay: PLCH1, Human
PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 45-bisphosphate (PtdIns(45)P2) to generate second messengers inositol 145-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al. 2005 [PubMed 15702972]).[supplied by OMIM Jun 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.