PrimePCR™ SYBR® Green Assay: NEK11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050822

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NEK11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NEK11, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043560
Assay Design:   exonic
Chromosome Location:   3:130748541-130748642question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000510769 ENST00000510688 ENST00000511262 ENST00000383366 ENST00000507910 ENST00000508196 ENST00000429253 ENST00000356918 ENST00000412440

Gene Information

This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.[provided by RefSeq Mar 2009]

Gene Symbol:   NEK11
Gene Name:   NIMA (never in mitosis gene a)- related kinase 11
Aliases:   FLJ23495
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114670
Entrez:   79858
Chromosome Mapping:   3q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.200000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download