PrimePCR™ SYBR® Green Assay: ABTB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050818

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ABTB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ABTB1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043556
Assay Design:   exonic
Chromosome Location:   3:127399641-127399755question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000232744 ENST00000453791 ENST00000468137 ENST00000393363

Gene Information

This gene encodes a protein with an ankyrin repeat region and two BTB/POZ domains which are thought to be involved in protein-protein interactions. Expression of this gene is activated by the phosphatase and tensin homolog a tumor suppressor. Alternate splicing results in three transcript variants. [provided by RefSeq Mar 2010]

Gene Symbol:   ABTB1
Gene Name:   ankyrin repeat and BTB (POZ) domain containing 1
Aliases:   BPOZ, BTB3, EF1ABP, MGC20585
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114626
Entrez:   80325
Chromosome Mapping:   3q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.580000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download