This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050801
PrimePCR™ PreAmp for SYBR® Green Assay: SERPINI2, Human
PrimePCR™ Template for SYBR® Green Assay: SERPINI2, Human
The protein encoded by this gene is a member of the serine protease inhibitor (serpin) superfamily made up of proteins which play central roles in the regulation of a wide variety of physiological processes including coagulation fibrinolysis development malignancy and inflammation. The gene product may have a role in a growth-control possibly growth-suppressing pathway and when impaired may be involved in pancreatic carcinogenesis. The protein is a member of the plasminogen activator inhibitor-1 family a subset of the serpin superfamily whose members act as tissue-specific tPA inhibitors. Two alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.