PrimePCR™ SYBR® Green Assay: TBCCD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050785

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TBCCD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TBCCD1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043523
Assay Design:   exonic
Chromosome Location:   3:186274418-186274525question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000331243 ENST00000295092 ENST00000354532 ENST00000599846 ENST00000347545 ENST00000337665 ENST00000258770 ENST00000361278 ENST00000395655 ENST00000494076 ENST00000432126 ENST00000338733 ENST00000446782 ENST00000413695 ENST00000424280 ENST00000300632 ENST00000337883 ENST00000418738 ENST00000381389 ENST00000381392 ENST00000434045 ENST00000416167 ENST00000381406 ENST00000381395

Gene Information

Description Not Available

Gene Symbol:   TBCCD1
Gene Name:   TBCC domain containing 1
Aliases:   FLJ10560
RefSeq:   NC_000003.11 NG_009829.1 NT_005612.16
Ensembl:   ENSG00000113838
Entrez:   55171
Chromosome Mapping:   3q27.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.230000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download