PrimePCR™ SYBR® Green Assay: TTC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050749

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043487
Assay Design:   exonic
Chromosome Location:   5:159476546-159478146question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000522793 ENST00000231238 ENST00000518560

Gene Information

Description Not Available

Gene Symbol:   TTC1
Gene Name:   tetratricopeptide repeat domain 1
Aliases:   FLJ46404, TPR1
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000113312
Entrez:   7265
UniGene:   Hs.519718
Chromosome Mapping:   5q33.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.760000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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