PrimePCR™ SYBR® Green Assay: PFDN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050729

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PFDN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PFDN1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043467
Assay Design:   exonic
Chromosome Location:   5:139624878-139625018question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000510217 ENST00000261813

Gene Information

This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides thereby allowing them to fold correctly. The complex consisting of two alpha and four beta subunits forms a double beta barrel assembly with six protruding coiled-coils. [provided by RefSeq Jul 2008]

Gene Symbol:   PFDN1
Gene Name:   prefoldin subunit 1
Aliases:   PDF, PFD1
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000113068
Entrez:   5201
Chromosome Mapping:   5q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.600000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download