PrimePCR™ SYBR® Green Assay: WISP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050718

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043456
Assay Design:   exonic
Chromosome Location:   6:112389599-112390672question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000368666 ENST00000368663 ENST00000409166 ENST00000361714 ENST00000230529 ENST00000584854 ENST00000582958 ENST00000581324 ENST00000580286 ENST00000541400

Gene Information

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain von Willebrand factor type C module thrombospondin domain and C-terminal cystine knot-like domain. This gene is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of this gene are associated with progressive pseudorheumatoid dysplasia an autosomal recessive skeletal disorder indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   WISP3
Gene Name:   WNT1 inducible signaling pathway protein 3
Aliases:   CCN6, LIBC, MGC125987, MGC125988, MGC125989, PPAC, PPD
RefSeq:   NC_000006.11 NG_011748.1 NT_025741.15
Ensembl:   ENSG00000112761
Entrez:   8838
UniGene:   Hs.558428
Chromosome Mapping:   6q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.460000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download