PrimePCR™ SYBR® Green Assay: PRPF4B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050717

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043455
Assay Design:   exonic
Chromosome Location:   6:4031949-4032086question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000337659 ENST00000538861

Gene Information

Pre-mRNA splicing occurs in two sequential transesterification steps and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq Jul 2008]

Gene Symbol:   PRPF4B
Gene Name:   PRP4 pre-mRNA processing factor 4 homolog B (yeast)
Aliases:   KIAA0536, PR4H, PRP4, PRP4H, PRP4K, dJ1013A10.1
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000112739
Entrez:   8899
UniGene:   Hs.159014
Chromosome Mapping:   6p25.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.660000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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