PrimePCR™ SYBR® Green Assay: CUL9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050711

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043449
Assay Design:   exonic
Chromosome Location:   6:43155170-43155571question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000252050 ENST00000372647 ENST00000354495

Gene Information

Description Not Available

Gene Symbol:   CUL9
Gene Name:   cullin 9
Aliases:   DKFZp686G1042, DKFZp686P2024, H7AP1, PARC, RP3-330M21.2
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000112659
Entrez:   23113
UniGene:   Hs.485434
Chromosome Mapping:   6p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.480000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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