PrimePCR™ SYBR® Green Assay: QKI, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050705

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: QKI, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: QKI, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043443
Assay Design:   exonic
Chromosome Location:   6:163899816-163956016question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000453779 ENST00000275262 ENST00000392127 ENST00000361752 ENST00000361195 ENST00000424802 ENST00000544436 ENST00000537041 ENST00000544823

Gene Information

The protein encoded by this gene is an RNA-binding protein that regulates pre-mRNA splicing export of mRNAs from the nucleus protein translation and mRNA stability. The encoded protein is involved in myelinization and oligodendrocyte differentiation and may play a role in schizophrenia. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Sep 2011]

Gene Symbol:   QKI
Gene Name:   quaking homolog, KH domain RNA binding (mouse)
Aliases:   DKFZp586I0923, Hqk, QK, QK1, QK3
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000112531
Entrez:   9444
Chromosome Mapping:   6q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.120000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download