PrimePCR™ SYBR® Green Assay: PACRG, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050704

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PACRG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PACRG, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043442
Assay Design:   exonic
Chromosome Location:   6:163483333-163510418question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000337019 ENST00000366889 ENST00000366888 ENST00000534958 ENST00000542936

Gene Information

This gene encodes a protein that is conserved across metazoans. In vertebrates this gene is linked in a head-to-head arrangement with the adjacent parkin gene which is associated with autosomal recessive juvenile Parkinson's disease. These genes are co-regulated in various tissues and they share a bi-directional promoter. Both genes are associated with susceptibility to leprosy. The parkin co-regulated gene protein forms a large molecular complex with chaperones including heat shock proteins 70 and 90 and chaperonin components. This protein is also a component of Lewy bodies in Parkinson's disease patients and it suppresses unfolded Pael receptor-induced neuronal cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   PACRG
Gene Name:   PARK2 co-regulated
Aliases:   FLJ32724, GLUP, HAK005771, PARK2CRG, RP3-495O10.2
RefSeq:   NG_008289.1 NC_000006.11 NT_025741.15 NG_011525.1
Ensembl:   ENSG00000112530
Entrez:   135138
Chromosome Mapping:   6q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996400
y-intercept 36.000000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download