PrimePCR™ SYBR® Green Assay: PTP4A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050692

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PTP4A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PTP4A1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043430
Assay Design:   exonic
Chromosome Location:   6:64289983-64290092question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000370650 ENST00000370644 ENST00000370651

Gene Information

The protein encoded by this gene belongs to a small class of prenylated protein tyrosine phosphatases (PTPs) which contains a PTP domain and a characteristic C-terminal prenylation motif. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. This tyrosine phosphatase is a nuclear protein but may primarily associate with plasma membrane. The surface membrane association of this protein depends on its C-terminal prenylation. Overexpression of this gene in mammalian cells conferred a transformed phenotype which implicated its role in the tumorigenesis. Studies in rat suggested that this gene may be an immediate-early gene in mitogen-stimulated cells. [provided by RefSeq Jul 2008]

Gene Symbol:   PTP4A1
Gene Name:   protein tyrosine phosphatase type IVA, member 1
Aliases:   DKFZp779M0721, HH72, PRL-1, PRL1, PTP(CAAX1), PTPCAAX1
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000112245
Entrez:   7803
Chromosome Mapping:   6q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.220000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download