PrimePCR™ SYBR® Green Assay: HINT3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050676

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043414
Assay Design:   exonic
Chromosome Location:   6:126300175-126300278question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000229633

Gene Information

Histidine triad proteins such as HINT3 are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner 2002 [PubMed 12119013]).[supplied by OMIM Mar 2008]

Gene Symbol:   HINT3
Gene Name:   histidine triad nucleotide binding protein 3
Aliases:   FLJ33126, FLJ99898, HINT4, MGC22976
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000111911
Entrez:   135114
UniGene:   Hs.72325
Chromosome Mapping:   6q22.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.630000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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