PrimePCR™ SYBR® Green Assay: ASF1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050673

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043411
Assay Design:   exonic
Chromosome Location:   6:119215595-119221957question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000229595

Gene Information

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4 and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq Jul 2008]

Gene Symbol:   ASF1A
Gene Name:   ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Aliases:   CGI-98, CIA, DKFZp547E2110, HSPC146
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000111875
Entrez:   25842
UniGene:   Hs.292316
Chromosome Mapping:   6q22.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.510000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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