This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050653
PrimePCR™ PreAmp for SYBR® Green Assay: PTPN6, Human
PrimePCR™ Template for SYBR® Green Assay: PTPN6, Human
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitotic cycle and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains which act as protein phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene which encode distinct isoforms have been reported. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.