PrimePCR™ SYBR® Green Assay: COPS7A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050650

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043388
Assay Design:   exonic
Chromosome Location:   12:6837392-6838424question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000543155 ENST00000229251 ENST00000539735 ENST00000538410 ENST00000544725 ENST00000534947 ENST00000541866 ENST00000534877 ENST00000538753

Gene Information

Description Not Available

Gene Symbol:   COPS7A
Gene Name:   COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis)
Aliases:   CSN7A, MGC110877
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000111652
Entrez:   50813
UniGene:   Hs.530823
Chromosome Mapping:   12p13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.530000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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