PrimePCR™ SYBR® Green Assay: TIMELESS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050644

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043382
Assay Design:   exonic
Chromosome Location:   12:56811420-56811549question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000554616 ENST00000553532 ENST00000229201

Gene Information

Description Not Available

Gene Symbol:   TIMELESS
Gene Name:   timeless homolog (Drosophila)
Aliases:   FLJ12640, FLJ20714, TIM, TIM1, hTIM
RefSeq:   NC_000012.11 NG_021397.1 NT_029419.12
Ensembl:   ENSG00000111602
Entrez:   8914
UniGene:   Hs.118631
Chromosome Mapping:   12q12-q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.000000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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