PrimePCR™ SYBR® Green Assay: FZD10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0050636

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043374
Assay Design:   exonic
Chromosome Location:   12:130649447-130649550question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000539839 ENST00000229030

Gene Information

This gene is a member of the frizzled gene family. Members of this family encode 7-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Using array analysis expression of this intronless gene is significantly up-regulated in two cases of primary colon cancer. [provided by RefSeq Jul 2008]

Gene Symbol:   FZD10
Gene Name:   frizzled homolog 10 (Drosophila)
Aliases:   CD350, FZ-10, Fz10, FzE7, hFz10
RefSeq:   NC_000012.11 NT_009755.19
Ensembl:   ENSG00000111432
Entrez:   11211
UniGene:   Hs.733275
Chromosome Mapping:   12q24.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.160000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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