PrimePCR™ SYBR® Green Assay: ACAD10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050627

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ACAD10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ACAD10, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043365
Assay Design:   exonic
Chromosome Location:   12:112123900-112124015question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000329905 ENST00000304062 ENST00000505203 ENST00000259339 ENST00000427860 ENST00000474759 ENST00000490478 ENST00000415676 ENST00000288197 ENST00000514615 ENST00000509936 ENST00000413681 ENST00000549590 ENST00000455480 ENST00000552965 ENST00000507135 ENST00000515283 ENST00000313698 ENST00000392636

Gene Information

This gene encodes a member of the acyl-CoA dehydrogenase family of enzymes (ACADs) which participate in the beta-oxidation of fatty acids in mitochondria. The encoded enzyme contains a hydrolase domain at the N-terminal portion a serine/threonine protein kinase catlytic domain in the central region and a conserved ACAD domain at the C-terminus. Several alternatively spliced transcript variants of this gene have been described but the full-length nature of some of these variants has not been determined. [provided by RefSeq Nov 2008]

Gene Symbol:   ACAD10
Gene Name:   acyl-CoA dehydrogenase family, member 10
Aliases:   MGC5601
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000111271
Entrez:   80724
Chromosome Mapping:   12q24.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.540000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download