PrimePCR™ SYBR® Green Assay: MDK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050579

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MDK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MDK, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043317
Assay Design:   exonic
Chromosome Location:   11:46404288-46405149question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000405308 ENST00000359803 ENST00000395569 ENST00000395566 ENST00000407067 ENST00000395565

Gene Information

Midkine is a retinoic acid-responsive heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis cell growth and cell migration. Midkine is also expressed in several carcinomas suggesting that it may play a role in tumorigenesis perhaps through its effects on angiogenesis (summary by Reynolds et al. 2004 [PubMed 15197188]).[supplied by OMIM Nov 2010]

Gene Symbol:   MDK
Gene Name:   midkine (neurite growth-promoting factor 2)
Aliases:   FLJ27379, MK, NEGF2
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000110492
Entrez:   4192
Chromosome Mapping:   11p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.750000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download