This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0050548
PrimePCR™ PreAmp for SYBR® Green Assay: CLNK, Human
PrimePCR™ Template for SYBR® Green Assay: CLNK, Human
MIST is a member of the SLP76 family of adaptors (see LCP2 MIM 601603; BLNK MIM 604515). MIST plays a role in the regulation of immunoreceptor signaling including PLC-gamma (PLCG1; MIM 172420)-mediated B cell antigen receptor (BCR) signaling and FC-epsilon R1 (see FCER1A MIM 147140)-mediated mast cell degranulation (Cao et al. 1999 [PubMed 10562326]; Goitsuka et al. 2000 2001 [PubMed 10744659] [PubMed 11463797]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.