This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0050540
PrimePCR™ PreAmp for SYBR® Green Assay: WFS1, Human
PrimePCR™ Template for SYBR® Green Assay: WFS1, Human
This gene encodes a transmembrane protein which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain pancreas heart and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome also called DIDMOAD (Diabetes Insipidus Diabetes Mellitus Optic Atrophy and Deafness) an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6) also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Mar 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.