PrimePCR™ SYBR® Green Assay: KLHL2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050538

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KLHL2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KLHL2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043276
Assay Design:   exonic
Chromosome Location:   4:166160048-166184448question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000308230 ENST00000382179 ENST00000528710 ENST00000524702 ENST00000526678 ENST00000531197 ENST00000528841 ENST00000308219 ENST00000314616 ENST00000347486 ENST00000361852 ENST00000369805 ENST00000361965 ENST00000434735 ENST00000342494 ENST00000538070 ENST00000550890 ENST00000011653 ENST00000226725 ENST00000509704 ENST00000514860 ENST00000421009 ENST00000506761 ENST00000538127 ENST00000594128

Gene Information

Description Not Available

Gene Symbol:   KLHL2
Gene Name:   kelch-like 2, Mayven (Drosophila)
Aliases:   ABP-KELCH, MAV, MAYVEN
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000109466
Entrez:   11275
Chromosome Mapping:   4q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.710000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download