PrimePCR™ SYBR® Green Assay: USP46, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050531

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043269
Assay Design:   exonic
Chromosome Location:   4:53492271-53492393question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000441222 ENST00000451218 ENST00000508499

Gene Information

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al. 2004 [PubMed 14715245]).[supplied by OMIM Jun 2009]

Gene Symbol:   USP46
Gene Name:   ubiquitin specific peptidase 46
Aliases:   FLJ11850, FLJ12552, FLJ14283, FLJ39393
RefSeq:   NC_000004.11 NT_022853.15
Ensembl:   ENSG00000109189
Entrez:   64854
UniGene:   Hs.7966
Chromosome Mapping:   4q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.700000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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