PrimePCR™ SYBR® Green Assay: RAB34, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050526

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RAB34, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RAB34, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043264
Assay Design:   exonic
Chromosome Location:   17:27042839-27043033question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000447716 ENST00000419712 ENST00000412625 ENST00000353676 ENST00000430132 ENST00000436730 ENST00000395245 ENST00000395242 ENST00000415040 ENST00000450529 ENST00000301043 ENST00000453384 ENST00000395243

Gene Information

This gene encodes a protein belonging to the RAB family of proteins which are small GTPases involved in protein transport. This family member is a Golgi-bound member of the secretory pathway that is involved in the repositioning of lysosomes and the activation of macropinocytosis. Alternative splicing of this gene results in multiple transcript variants. This gene overlaps and shares exon structure with the nine-amino acid residue-repeats (NARR) gene which encodes a functionally distinct nucleolar protein from a different reading frame. [provided by RefSeq Jan 2012]

Gene Symbol:   RAB34
Gene Name:   RAB34, member RAS oncogene family
Aliases:   RAB39, RAH
RefSeq:   NC_000017.10 NT_010799.15
Ensembl:   ENSG00000109113
Entrez:   83871
Chromosome Mapping:   17q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.450000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download