PrimePCR™ SYBR® Green Assay: LRRC59, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050515

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043253
Assay Design:   exonic
Chromosome Location:   17:48474593-48474688question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000445041 ENST00000555262 ENST00000449574 ENST00000339223 ENST00000483320 ENST00000215906 ENST00000419228 ENST00000435085 ENST00000307198 ENST00000338432 ENST00000377848 ENST00000377854 ENST00000543201 ENST00000262715 ENST00000556935 ENST00000462833 ENST00000311895 ENST00000356082 ENST00000367030 ENST00000391911 ENST00000576448 ENST00000225972 ENST00000264870 ENST00000291906 ENST00000304129 ENST00000369271 ENST00000419268 ENST00000545353 ENST00000372492

Gene Information

Description Not Available

Gene Symbol:   LRRC59
Gene Name:   leucine rich repeat containing 59
Aliases:   FLJ21675
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000108829
Entrez:   55379
UniGene:   Hs.370927
Chromosome Mapping:   17q21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.560000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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