PrimePCR™ SYBR® Green Assay: ABI3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050508

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043246
Assay Design:   exonic
Chromosome Location:   17:47300277-47300390question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000225941 ENST00000419580 ENST00000571035

Gene Information

This gene encodes a member of an adaptor protein family. Members of this family encode proteins containing a homeobox homology domain proline rich region and Src-homology 3 (SH3) domain and are components of the Abi/WAVE complex which regulates Rac-dependent actin polymerization. The encoded protein inhibits ectopic metastasis of tumor cells as well as cell migration. This may be accomplished through interaction with p21-activated kinase. [provided by RefSeq Sep 2008]

Gene Symbol:   ABI3
Gene Name:   ABI family, member 3
Aliases:   NESH, SSH3BP3
RefSeq:   NC_000017.10 NG_009841.1 NT_010783.15
Ensembl:   ENSG00000108798
Entrez:   51225
UniGene:   Hs.130719
Chromosome Mapping:   17q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.620000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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