This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0050507
PrimePCR™ PreAmp for SYBR® Green Assay: CNTNAP1, Human
PrimePCR™ Template for SYBR® Green Assay: CNTNAP1, Human
The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. The 1384-amino acid protein also designated p190 or CASPR for 'contactin-associated protein' includes an extracellular domain with several putative protein-protein interaction domains a putative transmembrane domain and a 74-amino acid cytoplasmic domain. Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb with weak expression in several other tissues tested. The architecture of its extracellular domain is similar to that of neurexins and this protein may be the signaling subunit of contactin enabling recruitment and activation of intracellular signaling pathways in neurons. [provided by RefSeq Jan 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.