PrimePCR™ SYBR® Green Assay: DHX40, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050486

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043224
Assay Design:   exonic
Chromosome Location:   17:57650578-57651227question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000251241 ENST00000425628 ENST00000584385 ENST00000580030 ENST00000451169

Gene Information

This gene encodes a member of the DExH/D box family of ATP-dependent RNA helicases that have an essential role in RNA metabolism. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 17.[provided by RefSeq Oct 2009]

Gene Symbol:   DHX40
Gene Name:   DEAH (Asp-Glu-Ala-His) box polypeptide 40
Aliases:   DDX40, FLJ22060, FLJ34015, PAD
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000108406
Entrez:   79665
UniGene:   Hs.29403
Chromosome Mapping:   17q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 36.600000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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