PrimePCR™ SYBR® Green Assay: RNF43, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050483

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RNF43, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RNF43, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043221
Assay Design:   exonic
Chromosome Location:   17:56435631-56435733question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000500597 ENST00000581868 ENST00000577625 ENST00000577716 ENST00000584437 ENST00000583753 ENST00000407977

Gene Information

RNF43 is a HAP95 (AKAP8L; MIM 609475) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer (Yagyu et al. 2004 [PubMed 15492824]; Sugiura et al. 2008 [PubMed 18313049]).[supplied by OMIM Dec 2008]

Gene Symbol:   RNF43
Gene Name:   ring finger protein 43
Aliases:   DKFZp781H02126, DKFZp781H0392, FLJ20315, FLJ77466, FLJ99338, MGC125630, RNF124, URCC
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000108375
Entrez:   54894
Chromosome Mapping:   17q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.130000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download