PrimePCR™ SYBR® Green Assay: PITRM1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050469

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043207
Assay Design:   exonic
Chromosome Location:   10:3185604-3186489question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000224949 ENST00000380994 ENST00000455371 ENST00000451454 ENST00000451104 ENST00000380989

Gene Information

Description Not Available

Gene Symbol:   PITRM1
Gene Name:   pitrilysin metallopeptidase 1
Aliases:   KIAA1104, MGC138192, MGC141929, MP1, PreP, hMP1
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000107959
Entrez:   10531
UniGene:   Hs.528300
Chromosome Mapping:   10p15.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.100000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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