PrimePCR™ SYBR® Green Assay: PITX3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0050461

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043199
Assay Design:   exonic
Chromosome Location:   10:103990857-103991451question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000389201 ENST00000599957 ENST00000507253 ENST00000503586 ENST00000506438 ENST00000265340 ENST00000409379 ENST00000417669 ENST00000569563 ENST00000533211 ENST00000529997 ENST00000309996 ENST00000342992 ENST00000460472 ENST00000589042 ENST00000591111 ENST00000360870 ENST00000342175 ENST00000359218 ENST00000334456 ENST00000376450 ENST00000544570 ENST00000444035 ENST00000253928 ENST00000416288 ENST00000399667 ENST00000328939 ENST00000520740 ENST00000434108 ENST00000411494 ENST00000390656 ENST

Gene Information

This gene encodes a member of the RIEG/PITX homeobox family which is in the bicoid class of homeodomain proteins. Members of this family act as transcription factors. This protein is involved in lens formation during eye development. Mutations of this gene have been associated with anterior segment mesenchymal dysgenesis and congenital cataracts. [provided by RefSeq Jul 2008]

Gene Symbol:   PITX3
Gene Name:   paired-like homeodomain 3
Aliases:   CTPP4, MGC12766, PTX3
RefSeq:   NC_000010.10 NG_008147.1 NT_030059.13
Ensembl:   ENSG00000107859
Entrez:   5309
UniGene:   Hs.137568
Chromosome Mapping:   10q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.800000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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