PrimePCR™ SYBR® Green Assay: EIF3A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050454

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043192
Assay Design:   exonic
Chromosome Location:   10:120795461-120795608question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000369144 ENST00000541549

Gene Information

Description Not Available

Gene Symbol:   EIF3A
Gene Name:   eukaryotic translation initiation factor 3, subunit A
Aliases:   EIF3, EIF3S10, KIAA0139, P167, TIF32, eIF3-p170, eIF3-theta, p180, p185
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000107581
Entrez:   8661
UniGene:   Hs.523299
Chromosome Mapping:   10q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.510000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download