This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0050428
PrimePCR™ PreAmp for SYBR® Green Assay: SLC1A1, Human
PrimePCR™ Template for SYBR® Green Assay: SLC1A1, Human
This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate and mutations in this gene are thought to cause dicarboxylicamino aciduria also known as glutamate-aspartate transport defect. [provided by RefSeq Mar 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.