PrimePCR™ SYBR® Green Assay: TAF6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050400

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043138
Assay Design:   exonic
Chromosome Location:   7:99711282-99711387question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000453269 ENST00000472509 ENST00000452041 ENST00000344095 ENST00000437822 ENST00000493322 ENST00000440225 ENST00000452438 ENST00000523306 ENST00000449571 ENST00000520135 ENST00000451699 ENST00000418432 ENST00000296503

Gene Information

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID) which binds to the core promoter to position the polymerase properly serves as the scaffold for assembly of the remainder of the transcription complex and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription serve as coactivators function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1 the largest subunit of TFIID. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jun 2010]

Gene Symbol:   TAF6
Gene Name:   TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa
Aliases:   DKFZp781E21155, MGC:8964, TAF(II)70, TAF(II)80, TAF2E, TAFII-70, TAFII-80, TAFII70, TAFII80, TAFII85
RefSeq:   NC_000007.13 NG_016312.1 NT_007933.15
Ensembl:   ENSG00000106290
Entrez:   6878
UniGene:   Hs.489309
Chromosome Mapping:   7q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.550000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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