PrimePCR™ SYBR® Green Assay: CRHR2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050393

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CRHR2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CRHR2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043131
Assay Design:   exonic
Chromosome Location:   7:30702345-30702458question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000471646 ENST00000348438 ENST00000341843 ENST00000506074

Gene Information

The protein encoded by this gene belongs to the G-protein coupled receptor 2 family and the subfamily of corticotropin releasing hormone receptor. This receptor shows high affinity for corticotropin releasing hormone (CRH) and also binds CRH-related peptides such as urocortin. CRH is synthesized in the hypothalamus and plays an important role in coordinating the endocrine autonomic and behavioral responses to stress and immune challenge. Studies in mice suggest that this receptor maybe involved in mediating cardiovascular homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq Jan 2011]

Gene Symbol:   CRHR2
Gene Name:   corticotropin releasing hormone receptor 2
Aliases:   CRF2, CRFR2
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000106113
Entrez:   1395
Chromosome Mapping:   7p14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.620000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download