This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0050354
PrimePCR™ PreAmp for SYBR® Green Assay: GSK3A, Human
PrimePCR™ Template for SYBR® Green Assay: GSK3A, Human
This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase and transcription factors such as JUN. It also plays a role in the WNT and PI3K signaling pathways as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.