PrimePCR™ SYBR® Green Assay: ERF, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050353

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ERF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ERF, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043091
Assay Design:   exonic
Chromosome Location:   19:42753992-42754091question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000598965 ENST00000593944 ENST00000440177 ENST00000222329 ENST00000343053

Gene Information

Members of the ETS family of transcription factors such as ERF regulate cell proliferation and differentiation. They share a highly conserved DNA-binding domain the ETS domain that recognizes the sequence GGAA/T (de Castro et al. 1997 [PubMed 9192842]). For further information on ETS transcription factors see ETS1 (MIM 164720).[supplied by OMIM Mar 2008]

Gene Symbol:   ERF
Gene Name:   Ets2 repressor factor
Aliases:   PE-2, PE2
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000105722
Entrez:   2077
Chromosome Mapping:   19q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.430000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download