PrimePCR™ SYBR® Green Assay: SUGP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050352

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SUGP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SUGP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043090
Assay Design:   exonic
Chromosome Location:   19:19420906-19420996question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000389840 ENST00000585328 ENST00000324695 ENST00000456930 ENST00000433712 ENST00000550422 ENST00000307885 ENST00000357869 ENST00000244763 ENST00000397511 ENST00000489567 ENST00000534851 ENST00000371929 ENST00000355699 ENST00000356589 ENST00000371916 ENST00000268876 ENST00000394570 ENST00000433649 ENST00000378449 ENST00000465301 ENST00000540313 ENST00000308508 ENST00000247001 ENST00000535070 ENST00000334782 ENST00000276297 ENST00000316609 ENST00000511869 ENST00000333493 ENST00000450388 ENST

Gene Information

SF4 is a member of the SURP family of splicing factors.[supplied by OMIM Sep 2003]

Gene Symbol:   SUGP1
Gene Name:   SURP and G patch domain containing 1
Aliases:   DKFZp434E2216, F23858, RBP, SF4
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000105705
Entrez:   57794
Chromosome Mapping:   19p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.550000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download