PrimePCR™ SYBR® Green Assay: SIGLEC8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050322

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SIGLEC8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SIGLEC8, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043060
Assay Design:   exonic
Chromosome Location:   19:51961405-51961501question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000430817 ENST00000321424 ENST00000340550

Gene Information

Sialic acid-binding immunoglobulin (Ig)-like lectins or SIGLECs (e.g. CD33 (MIM 159590)) are a family of type 1 transmembrane proteins each having a unique expression pattern mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif or ITIM (see MIM 604964) and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al. 2000 [PubMed 11095983]).[supplied by OMIM May 2010]

Gene Symbol:   SIGLEC8
Gene Name:   sialic acid binding Ig-like lectin 8
Aliases:   MGC59785, SAF2, SIGLEC-8, SIGLEC8L
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000105366
Entrez:   27181
Chromosome Mapping:   19q13.33-q13.41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.440000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download