PrimePCR™ SYBR® Green Assay: LYL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050301

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LYL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LYL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043039
Assay Design:   exonic
Chromosome Location:   19:13213415-13213545question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000576946 ENST00000574943 ENST00000253686 ENST00000264824 ENST00000590974 ENST00000450633 ENST00000355552 ENST00000489960 ENST00000359486 ENST00000335626 ENST00000361573 ENST00000417961 ENST00000221494 ENST00000372126 ENST00000377948 ENST00000457542 ENST00000456763 ENST00000221214 ENST00000260357 ENST00000268893 ENST00000343736 ENST00000355701 ENST00000220244 ENST00000394685 ENST00000356249 ENST00000549260 ENST00000303694 ENST00000409251 ENST00000295718

Gene Information

This gene represents a basic helix-loop-helix transcription factor. The encoded protein may play roles in blood vessel maturation and hematopoeisis. A translocation between this locus and the T cell receptor beta locus (GeneID 6957) on chromosome 7 has been associated with acute lymphoblastic leukemia. [provided by RefSeq Sep 2010]

Gene Symbol:   LYL1
Gene Name:   lymphoblastic leukemia derived sequence 1
Aliases:   bHLHa18
RefSeq:   NC_000019.9 NG_027756.1 NT_011295.11
Ensembl:   ENSG00000104903
Entrez:   4066
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 36.320000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download